Stress proteins expressed in Pseudomonas aeruginosa on exposure to methomyl

Amritha G Kulkarni, Basappa B Kaliwal

Abstract


Prokaryotic cells respond to environmental or chemical stress by inducing specific sites of proteins characteristic to each stress. Hence, Pseudomonas aeruginosa, a gram –ve bacillus is one of the most popular model used for studying, roles of stress, owing to its duplication time and rapid response to the toxicants. Pseudomonas aeruginosa was exposed to methomyl at concentrations ranging from 10-7 M to 10-3 M for a period of 96 hrs and protein profiling was elucidated at regular intervals of 24, 48, 72 and 96 hrs along with the control and standard markers. . The protein expression was observed at 19, 33, 38, 58, 85 and 115 kDa at 24 hrs, 16, 19, 33, 38, 58, 78 115 kDa, at 48 hrs, 19, 38, 58, 85, 97 and 115 kDa at 72 hrs 19, 33, 38, 57, 97 and 115 kDa  at 96 hrs respectively. The stress proteins induced in Pseudomonas aeruginosa in response to methomyl indicated that it was dose and durational dependent. Upon comparison of the pesticide-induced proteins, it is apparent that nature of the stress remained the same throughout, which indirectly proves that the genes responsible for the stress expression are the same, irrespective of the chemical nature of the substituents of the pesticide. There are many proteins expressed in common with the stress induced by methomyl in Pseudomonas aeruginosa. Some of these proteins may play a major role in the stability of the cells under different stress.


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ISSN : 2251-1563