Isolation and Identification of Oral flora from individuals belonging to ages 7 to 16 years

Kutcharlapati Sunita Raju, Layam Anitha


Dental caries being one of the most prevalent oral diseases persists to be an important public health problem worldwide. It is observed in both the sexes, all races, all socioeconomic classes and all age groups. In the developing countries like India, the prevalence of dental caries is very high particularly among the children and adolescents. Dental caries in an individual in permanent dentition is directly related to the oral microflora established with completion of primary dentition. Dental caries is a process where the enamel and the dentine are demineralised by the products of bacterial fermentation. It is an infectious process that ends up in the destruction of hard dental tissue. It results from the accumulation of plaque on the surface of the teeth and biochemical activities of complex microorganisms. Streptococcus mutans is one of the main opportunistic pathogen of dental caries, which plays a main role in fermenting carbohydrates that result in the  production of acids, leading to the corrosion of tooth enamel. In addition, other micro flora like Lactobacillus species, Bacillus cereus, Micrococcus luteus, Pseudomonas aeruginosa, and Staphylococcus aureus are also associated with dental caries. In the present study isolation of the microorganisms was done from the teeth and gums of children belonging to age group 7 to 16 years. Identification  of the organisms was done by physical and biochemical characterization. Micrococcus luteus and Bacillus subtilis were the most predominant bacteria among all isolates (17.85%) followed by Lactobacillus acidophilus and Streptococcus oralis (14.28%). Bacillus cereus (10.71%), Streptococcus mutans and Staphylococcus aureus (7.14% ) and Escherichia coli, Enterobacter and Pseudomonas aeruginosa (3.57%) were also isolated.

Keywords: Dental caries, oral microflora, isolation, identification, physical and biochemical characterization.



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