FORMULATION OF GUAVA FRUIT BEVERAGE WITH ISOLATED PROBIOTIC STRAINS

LAYAM ANITHA

Abstract


The suitability of Guava fruit beverage for the production of probiotic juice with Lactic acid bacteria isolated from milk, curd, whey and Lactobacillus plantarum has been studied. The probiotic strains isolated from dairy products are characterized morphologically and biochemically. The formulated Guava fruit beverage was inoculated with these isolates and incubated at 300C.  Changes in pH, acidity, sugar content, and viable cell counts during fermentation under controlled conditions were measured. The pH of the Guava fruit beverage was initially 5.5 at 300 C and decreased gradually to a suitable range. The titrable acidity at 300 C found to be 0.28% – 0.32% and was increased on further fermentation. The sugar at 300 C was initially 15-22g/ 100ml. The viable cell counts at 300C were estimated and they ranged between 5.6 x 10 6- 8.9x106. The fruit beverage was assessed for acceptability through sensory evaluation. Molecular confirmation of isolate no.8 through 16s rDNA sequence has been done and was found to be L. coryneformis. The final product resulted in suitable pH, acidity, sugar content and ideal number of viable cell counts. The Probiotic Guava fruit beverage could serve as a healthy beverage for consumers with dairy allergy, beneficial to gut health, prevention of diarrhea and best nutrient source for under nourished vulnerable population.

 

Key words: Probiotics, Fermentation, Guava fruit beverage, Lactic acid bacteria, Diarrhea.


References


Amal A. Hassan, Mona M, A. Aly and Soher T. El-Hadidie, (2013) ‘‘Production of Cereal-Based Probiotic Beverages’’ Int Food Res Journal 20(1): 7-15: http://www.ifrj.upm.edu.my

Anuradha, S, Rajeshwari, K. (2005). ‘‘Probiotics in health and disease’’. J Indian Acad Clin Med. 6, 67-72.

Atkins, J. P.; Mazzi, S.; Easter, C.D. (2000). ‘‘A Commonwealth Vulnerability Index for Developing Countries: The Position of Small States’’. Commonwealth Economic Paper Series 40, Commonwealth Secretariat, London, www.thecommonwealth.org/.../...

Black, R.; Cousens, S.; Johnson, H.L.; Lawn, J.E.; Rudan, I.; Bassani, D.G.; Et al. (2010). ‘‘Global, regional, and national causes of child mortality in 2008: a systematic analysis’’. The Lancet. 375, 1969-87.

Bloom and Canning, (2000). ‘‘Population and Economic growth available at’’ www.growthcommission.org/storage/cgdev/documents/gcwp024web.pdf

Ehrlich Paul. (1990) ‘‘the Population Explosion’’ p 153. Simon and Schuster, New York.

.

FAO/WHO (2002) ‘‘Guidelines for the evaluation of probiotics in food’’. FAO website: ftp://ftp.fao.org/es/esn/food/ wgreport2.pdf. Accessed 8 Apr 2010.

Fuller, R. (1989). ‘‘Probiotics in man and animals’’. J. of Appl. Bacteriol 66, 365-37.

Gorbach, S.L. (2000). ‘‘Probiotics and gastrointestinal health’’. American J. of Gastroent, Vol 95, Iss 1, Suppl. S, pp S2-S4.

James Cuppucino and Natalie Sherman, (2004). MICROBIOLOGY A LABORATORY MANUAL Pearson Education (US) Benjamin-Cummings Publishing Company, Subs of Addison Wesley Longman, Inc 05 Apr 2004.

Kyung Young Yoon; Edward, E.; Woodams, L.; Yong D Hang, L. (2004). December ‘‘Probiotication of Tomato Juice by Lactic Acid Bacteria’’ The J.of Microbio, p.315-318.

Kyung Young Yoon; Edward, E.; Woodams, L.; Yong D Hang, L. (2005). June, ‘‘Production of probiotic cabbage juice by lactic acid bacteria’’. Biores tech, Vol. 97, No. 12. (August 2006), pp. 1427-1430, doi:10.1016/j.biortech.2005.06.018

Lilly, D.M.; Stillwell, R.H. (1965). ‘‘Probiotics: growth-promoting factors produced by microorganisms’’. Science; 147,747-8.

Lin Kiat Saw, Suijing Chen, Siew Hwa Wong, Soon Ann Tan, Kelvin K.T Goh, 2011. ‘‘The 12th ASEAN FOOD CONFERENCE 2011’’ 16 -18 June, 2011 BITEC Bangna, Bangkok, Thailand.

Lochery, P. (2005). ‘‘The impact of water and sanitation on the health of children under five: Global Health Council’’ available at 217.218.200.220/documents/ 10129/20978/02-Feb.pdf?version=1...

Mahrosh naeem, M. Ilyas, Saleem haider, Shahjahan baig and m. Saleem, (2012). “Isolation characterization and identification of lactic acid bacteria from fruit juices and their efficacy against antibiotics”. Pak. J. Bot., 44: 323-328, Special Issue March 2012.

Metchnikoff, E. (1907). ‘‘In the Prolongation of Life. In: Optimistic Studies, Chalmers’’ M. (Ed.). William Heinemann, London

Mirzaei Hamid and Barzgari Abulfazl, (2012). “Isolation and Molecular Study of Potentially Probiotic Lactobacilli in Traditional White Cheese of Tabriz in Iran”. Annals of Biological Research, 2012, 3 (4):2019-2022 (http://scholarsres earchlibrary.com/archive.html)

Mohammad Daneshi, Mohammad Reza Ehsani, Seyed Hadi Razavi, Mohsen Labbaf (2013). “Effect of refrigerated storage on the probiotic survival and sensory properties of milk/carrot juice mix drink”. Electronic Journal of Biotechnology; ISSN: 0717-3458; http://www.ejbiotechnology.info ; DOI: 10.2225/vol16-issue5-fulltext-2; Received February 11, 2013 / Accepted June 22, 2013

Mortality and disease burden rate (2004), www.who.int/healthinfo/...burden disease/.

NFHS-3 (2005-06), www.measuredhs.com/pubs/pdf/FRIND3/FRIND3-VOL2.pdf

PS Panesar (2011), ‘‘Fermented Dairy Products: Starter Cultures and Potential Nutritional Benefits’’ PP. 47-51 DOI: 10.4236/fns.2011.2100 www.scirp.org/Journal/PaperInformation.aspx?paperID=3643

Pochapin, M. (2000). ‘‘The effect of Probiotics on Clostridium difficile diarrhea’’. American J.of Gastroent, Vol 95, Iss 1, Suppl. S, pp S11-S13.

Progress for Children Report (December 2007)-A Statistical Review, www.unicef.org/.../files/Progress_for_Children_No_6_revised.pdf

Rakesh Gehlot et al. 2011 January, Nutritional and Therapeutic Beverages from Guava, Beverage and Food world, hau.ernet.in/cobs/cfst.pdf

Renuka Goyal, Harish Dhingra, Pratima Bajpai and Navneet Joshi, (2October,2012) Characterization of the Lactobacillus isolated from different curd samples, African Journal of Biotechnology Vol.11(79), pp.14448-14452, http://www.academicjournals.org/AJB, Accepted 16 February, 2012,DOI: 10.5897/AJB11.310 ISSN 1684–5315.

Rogosa, M.; Mitchell, J. A.; Wiserman, R.F. (1951). ‘‘A selective medium for isolation and enumeration of oral and fecal’’ lactobacilli. J. of. Dental. Re. 30, 682.

Saarela, M.; Mogensen, G.; Fonden, R.; Matto, J.; Matilla-Sandholm,T. (2002). ‘‘ bacteria: safety, functional and technological properties’’. J. of Biotech.

Sambrook, J.; Fritsch, E.F.; Maniatis, T. (1989). ‘‘ Molecular Cloning: A Laboratory Manual’’. 2nd edition. New York: Cold Spring Harbor Laboratory Press;

Sujatha S Narayan, Sharmila Jalgaonkar, S Shahani, Vijaya N Kulkarni, SS Narayan (2010) ‘‘ Probiotics:Current trends in the treatment of diarrhea’’ www.hkmj.org/article_pdfs /hkm1006p213.pdf

Sunil Kumar Mishra, Pratibha Mishra, Mohit Saxena (1 April – June, 2012) ‘‘Probiotics: An Approach for Better Treatment’’, Volume 3 Issue 2 Page No. 1042, RJPBCS. www.rjpbcs.com/pdf/2012_3(2)/[122].pdf

Talat mehamood et.al, (2009), ‘‘Isolation and identification of wild strains of Lactic acid bacteria for yoghurt preparation from indigenous dahi’’, Pak jou of Nutr 8 (6) : 866-871, ISSN 1680 – 5194, Asian network of scientific information, 2009

Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599

Vasudha, S. and Mishra, H. N. (2011) ‘‘Non dairy probiotic beverages’’. Food and Nutr Sci, , 2, 47-51,doi:10.4236/fns.2011.21006. (http://www.SciRP.org/journal/fns) ,Copyright © 2011 SciRes. FNS

Verma, S., Paliya, B.S., Prasad, S., Chaudhary, H.S (2013) “Antimicrobial Activity of Probiotic Microorganisms from Probioticated Carrot Juice against Selective Pathogenic Strains. International Journal of Scientific & Engineering Research, Volume 4, Issue 8, August-2013 2130 ISSN 2229-5518

World Disasters Report (2002). Geneva: International Federation of Red Cross and Red Crescent Societies. www.ifrc.org/Global/ Publications/disasters/WDR/WDR2010-full.pdf

World Health Organization. Global burden of disease report: (2004 update; 2008). www.who.int/healthinfo/global_burden_disease/2004_report_update/.

WHO. (2002) Improving child health in the community. WHO/FCH/CAH/02.12. Geneva: WHO, www.coregroup.org/storage/ .../who_keyfamily practices evidence.pdf


پاراگلایدر Full Text: PDF

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

ISSN : 2251-1563