THE STUDY OF EFFECT USED PROTEOLYTIC ENZYME ON WASTEWATER OF LATEX HEVEABRASILIENSIS PROCESSING ON COCOA NIBS

muhammad anis

Abstract


The free amino acid profile of cocoa nibs as a result of hydrolysis by proteolytic enzyme from wastewater of latex Hevea brasiliensis processing on cocoa nibs were monitored for 8 hours. Pepsin enzyme is using as control. There are similarities profile of free amino acids produced by the enzyme from wastewater and the pepsin enzyme. If using proteolytic enzyme from wastewater, the acidic free amino acid concentration decreased by 4.739 %, whereas hydrophobic and other free amino acids increased by 10.257 % and 15.497 %, respectively.While the pepsin enzyme provide the decrease in the acidic free amino acids by 18.08%, and increase the hydrophobic and other amino acids by 11.24 % and 13.70 % respectively.

Key word: Free amino acid profile, acidic free amino acid, hydrophobic free amino acid.


References


Amin, I., S. Jinap, and B. Jamilah, Proteolytic activity (aspartic endoproteinase and carboxypeptidase) of cocoa bean during fermentation. Journal of the Science of Food and Agriculture, 76: (1998), 123–128

Ampon K, dan M. Salleh, Preliminary studies of acid protease and its inhibitor in latex of Hevea brasiliensis, Pertanika 13 (3), 415 - 418 (1990)

Anis, M, W. Wahab, H. Usman, Pirman. Isolation and characterization of Protease enzyme from wastewater of latex Hevea brasiliensis processing on casein substrate. Journal Adiwidia Vol. IV (1) 2012

Arima K, Uchikoba, Yonezawa H, Shimada M and Kaneda M, 2000. Cucumisin-like protease from the latex of Euphorbia supine; Phytochemistry 53 639–644

de Brito, E.S, N.H.P. Garcia, A.C. Amancio, A.L.P. Valente, G. F. Pini and F. Augusto, Effect of autoclaving cocoa nibs before roasting on the precursors of the Maillard reaction and pyrazines, International Journal of Food Science and Technology 2001, 36, 625-630

Guilloteau M, M. Lalaoi, S. Michaux, P. Bucheli and J. McCarthy, Identification and characterization of the major aspartic proteinase activity in Theobromacacao Seeds. J Sci Food Agric 85:549–562 (2005)

Gupta, R., Q.K. Beg,• P. Lorenz Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol (2002) 59:15–32

Hashim, P., S. Jinap, S. K. S Muhammad and A. Ali. Changes in free amino acid, peptide-N, sugar and pyrazine concentration during cocoa fermentation. J Sci Food Agric 1998, 78, 535-542

Hoffman, M., A W. Decho. Proteolytic enzymes in the marine bacterium Pseudoalteromonas atlantica: post-secretional activation and effects of environmental conditions. Aquat Microb Ecol 23: 29–39, 2000

Huang G., Ying T, Huo P and Jiang J. Purification and characterization of a protease from Thermophilic bacillus strain HS08. African Journal of Biotechnology Vol. 5 (24), 2006, 2433-2438

Jinap, S., Y. Ikrawan, J. Bakar, N. Saari, H.N. Lioe, Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous Carboxypeptidase. Journal of Food Science Volume 73, Issue 7 (2008), H141–H147

Leal Jr, G.A, L. H. Gomes, P. Efraim, F. C. A. Tavares and A. Figueira, Fermentation of cacao (Theobroma cacao L.) seeds with a hybrid Kluyveromycesmarxianus strain improved product quality attributes. FEMS Yeast Res 8 (2008) 788–798

Misnawi, S. Jinap, S. Nazamid, B. Jamilah, Activation of remaining key enzymes in dried under-fermented cocoa beans and its effect on aroma precursor formation. Food Chemistry 78 (2002) 407–417

Sánchez-Mundo, M.L, C. Bautista-Mu˜noz, M.E. Jaramillo-Flores Characterization of the exopeptidase activity existing in Theobroma cacao L.during germination, Process Biochemistry 45 (2010) 1156–1162

Shi-Qing Peng, Jia-Hong Zhu, Hui-Liang Li and Wei-Min Tian. Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis. J. Biosci. 33(5), December 2008, 681–690

Subroto, T, Protein yang terkait dengan patogenesis dari Hevea Brasiliensis Muell Arg (Isolasi, Struktur dan Fungsi), Chemistry Dissertation, Institut Teknologi Bandung (2000).

Thompson, S.S., Miller, K.B., Lopez, A.S., 2001. Cocoa and coffee. In: Doyle, M.J., Beuchat, L.R., Montville, T.J. (Eds.), Food Microbiology—Fundamentals and Frontiers. ASM Press, Washington, D.C., pp. 721–733

Voigt, J., B. Biehl, H. Heinrichs, S. Kamaruddin, G. G. Marsoner and A. Hugi. In-vitro formation of cocoa-specific aroma precursors: aroma-related peptides generated from cocoa-seed protein by co-operation of an aspartic endoprotease and a carboxypeptidase. Food Chemistry Volume 49, Issue 2, (1994a), 173-180

Voigt, J., H. Heinrichs, G. Voigt and B. Biehl, Cocoa-specific aroma precursors are generated by proteolytic digestion of the vicilin-like globulin of cocoa seeds. Food Chemistry 50 (1994b) 177-184

Wattanachote, L., Use of Polyethylene Graft Maleic Anhydride Film For Binding Extractable Protein from Natural Rubber, Thesis Faculty of Graduate Studies Mahidol University Thailand (2007)

Witono, Y., A.Am, A. Subagio dan S. B. Widjanarko, Pemurnian Parsial Enzim Protease dari Getah Tanaman Biduri (Calotropis gigantea) Menggunakan Ammonium Sulphat. Jurnal Teknologi Pertanian, Vol. 7 No. 1 20-26 (2006)


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