M Sjahrul, Andi Makkasau, Noor Jalaluddin


Growth patterns and the toxity metal ions Cd2+ on phytoplankton nannochloris in medium conwy. In this study, has investigated the interaction of metal ions Cd2+ conwy presented in the culture medium of phytoplankton types nannochloris. Culture series made ​​with and without the addition of metal ions Cd2+. Observation of the growth pattern will be determined parameters nannochloris specific growth rate, the percentage of growth inhibition, and toxicity testing. The results showed that the pattern of growth in the medium conwy nannochloris without the addition of metal ions Cd2+ (control) had the highest growth curve. In the culture medium were added metal ions Cd2+ 0.25 mg/L chart shows the same pattern of growth relative to the control. On the addition of metal ions Cd2+ concentrations above 0.25 mg/L, can lower specific growth rate nannochloris reinforced with rising prices spsifiknya growth inhibition (PGI). The results of the statistical analysis of the different test form on exposure to metal ions Cd2+ nannochloris indicates that the concentration of metal ions Cd2+, which does not affect the growth of nannochloris is at a concentration of 0.01 to 0.25 ppm, and the highest price that can be tolerated concentration (MTC) by nannochloris on concentration of 0.25 ppm and the concentration of metal ions Cd2+, which causes a decrease in the growth rate by 50% relative to the blank EC50 = 0.849 ppm.

Key Words: Nannochloris, Metal ion Cd2+, spesific growth inhibition (PGI).


Asada, K., 1999, The water cycle in chloroplast: Scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology 50: 601-639

Asada, M. and Takashaki M.,1987, Production and scavenging of active oxygen in photosynthesis. In Photoinhibition: Topics in photosynthesis (Kyle DJ, Osmond CB, Arntzen CJ)

Baryla A, Carrier P, Franck F, Coulomb C, Sahut C, Havaux M, 2001, Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmiumpolluted soil: causes and consequences for photosynthesis and growth. Planta, 212: 696–709

Burcu Kokturk, 2006, Cadmium uptake and antioxidative enzyme in durum wheat cultivars in respon to increasing Cd application, Thesis, School of Engineering and Natural Sciences, Sabancı University

Dafre´ AL, Sies H, Akerboom T., 1996, Protein S-thiolation and regulation of microsomal glutathione transferase activity by the glutathione redox couple. Arch Biochem Biophys, 332: 288–294

Dat J.F., Van Breusegem F., Vandenabeele S., Vranova E., Van Montagu M., Inze D., 2000, Dual action of active oxygen species during plant strees responses, Cell Mol. Life Sci. 57: 779-795.

Foster P.L., 1977, Copper exlusion as a mechanism of heavy metal tolerance in a greean alga. Nature, Lond., 269: 322-323.

Gadallah, M.A., 1995, Effects of cadmium and kinetin on chlorophyll content, saccahrides and dry matter accumulation in sunflower plants. Biol. Plant., 37: 233-240.

Halliwell B, Gutteridge, JMC, 1995, Free Radicals in Biology and Medicine. London: Clarendon Press

Hastuti W. dan Djunaidah, 1991, Spirolina dalam dunia perikanan, BPAP, Jepara.

Iannelli MA, Pietrini F, Fiore L, Petrilli L, Massacci A. 2002, Antioxidant response to cadmium in Phragmites australis plants. Plant Physiol Biochem 40: 977–982

Keyhani, E., Abdi-Oskoui, F., Attar, F. and Keyhani, J., 2006, DNA strand breaks by metal-induced oxygen radicals in purified Salmonella typhimurium DNA. Ann. N.Y. Acad. Sci. in Press.

Keyhani, J., Keyhani, E., Einollahi, N., Minai-Tehrani, D., and Zarchipour, S., 2003, Heterogeneous inhibition of horseradish peroxidase activity bya cadmium. Biochim. Biophys. Acta. 1621: 140-148.

Kneer, R. and Zenk, M.H., 1992, Phytochelatins protect plant enzymes from heavy metals poisioning, Phytochemistry, 31(8):2663-2667.

Liljas, A., 1972, Crystal structure of human carbonic anhydrase C. Nature New Biol.235: 131–137.

Loiffler Hochberger A, Grill E, Gekeler W, Winnacker E-L, Zenk MH, 1989, Termination of the phytochelatin synthase reaction through sequestration of heavy metals by the reaction product. FEBS Letters 258:42-46

Lue-Kim, H., Wozniak, P.C., dan Fletcher, R.A., 1980, "Cd Toxicity on Synchronous Populations Chlorella ellipsoidea", Can. J. Bot., 58: 1781-1788

Moore J.W. and Ramamoorthy S., 1984, Heavy metals in natural waters, applied monitoring and impact assessment; Springer-Verlag New York Inc.

Nurhamsiah. 2010. Bioakumulasi Ion Cr6+ oleh Fitolankton Laut Nannochloris dan Tetraselmis Chuii dengan Penambahan Glutation. Unhas,Tesis

Olmos E, Martínez-Solano JR, Piqueras A, Hellín E., 2003, Early steps in the oxidative burst induced by cadmium cultured tobacco cells (BY-2 line). Journal of Experimental Botany, 54:291-301

Palar, H., 1994 : Pencemaran dan Toksikologi Logam Berat, PT. Rineka Cipta, Jakarta

Parsons, T. R., Y. Maita and C. M. Lalli (1984): A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford.

Ramos I, Esteban E, Lucena JJ, Garate A (2002) Cadmium uptake and subcellular distribution in plants of Lactica sp. Cd-Mn interaction. Plant Sci, 162: 761–767

Rochaix J. D.,1995, Chlamydomonas reinhardtii as the photosynthetic yeast. Annu Rev Genet, 29: 209–230

Romero-Puertas MC, Rodriguez-Serrano M, Corpas FJ, Gomez M, Del Rio LA., 2004, Cadmium induced subcellular accumulation of O2.- and H2O2 in pea leaves. Plant Cell and Environment. 27:1122-1134

Sanita` di Toppi L, Gabbrielli R., 1999, Response to cadmium in higher plants. Environmental and Experimental Botany 41:105-130

Schüendübel A, Nikolova P, Rudolf C, Polle A., 2002, Cadmium and H2O2-induced oxidative stress in Populus canescens roots. Plant Physiology and Biochemistry 40: 577-584

Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A., 2001, Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol, 127: 887–898

Seafdec,1985, Prawn Hatchery design and Operational, Aquaculture Extention Manual No. 9, Aquaculture Department, Tigbauan, Iliolo, Philippines.

Wang J, and Evangelou VP., 1995, Metal tolerance aspects of plant cell wall and vacuole. In: Pessarakli M (ed) Handbook of plant and crop physiology. Marcel Dekker, Inc., New York.

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